Rabbit anti-Bub3 IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00123 maps to a region between residues 275 and 325 of human Bub3 using the numbering given in Swiss-Prot entry O43684 (GeneID 9184). Antibody was affinity purified using an epitope specific to Bub3 immobilized on solid support.
The epitope recognized by IHC-00123-T maps to a region between residues 275 and 325 of human Bub3 using the numbering given in Swiss-Prot entry O43684 (GeneID 9184).
Additional Product Information
Bub3 is an adaptor protein and component of the spindle checkpoint machinery. Bub3 and other spindle checkpoint proteins ensure that segregation does not proceed until all replicated chromatids are associated with a functional bipolar spindle. Bub3 forms a constitutive complex with Bub1. Bub3 has been demonstrated to also function as a transcriptional repressor in association with Cdc20 and histone deacetylases.
Alternate Names
BUB3 budding uninhibited by benzimidazoles 3 homolog; BUB3L; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog; hBUB3; mitotic checkpoint component; mitotic checkpoint protein BUB3; testicular tissue protein Li 27
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.