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Rabbit anti-Bub3 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
Bub3
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 278 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (50+ slides)

10 µl (5+ slides)

$131.00 $492.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Guinea Pig, Mouse, Horse, Dog, Pig, Rabbit, Bovine, Monkey, Rat
Antigen Species
Human
Concentration
250 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to Bub3 immobilized on solid support.

The epitope recognized by IHC-00123 maps to a region between residues 275 and 325 of human Bub3 using the numbering given in Swiss-Prot entry O43684 (GeneID 9184).
Antibody was affinity purified using an epitope specific to Bub3 immobilized on solid support.

The epitope recognized by IHC-00123-T maps to a region between residues 275 and 325 of human Bub3 using the numbering given in Swiss-Prot entry O43684 (GeneID 9184).
Country of Origin
USA

Additional Product Information

Bub3 is an adaptor protein and component of the spindle checkpoint machinery. Bub3 and other spindle checkpoint proteins ensure that segregation does not proceed until all replicated chromatids are associated with a functional bipolar spindle. Bub3 forms a constitutive complex with Bub1. Bub3 has been demonstrated to also function as a transcriptional repressor in association with Cdc20 and histone deacetylases.

9184
BUB3
BUB3, mitotic checkpoint protein
O43684
Mitotic checkpoint protein BUB3

Alternate Names

BUB3 budding uninhibited by benzimidazoles 3 homolog; BUB3L; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog; hBUB3; mitotic checkpoint component; mitotic checkpoint protein BUB3; testicular tissue protein Li 27

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.