Rabbit anti-CAP-D2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-601A maps to a region between residue 1350 and the C-terminus (residue 1401) of human Chromosome Associated Protein D2 (Chromosome Condensation-Related SMC-Associated Protein 1) using the numbering given in entry NP_055680.2 (GeneID 9918).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CAP-D2 immobilized on solid support.
The epitope recognized by A300-601A-T maps to a region between residue 1350 and the C-terminus (residue 1401) of human Chromosome Associated Protein D2 (Chromosome Condensation-Related SMC-Associated Protein 1) using the numbering given in entry NP_055680.2 (GeneID 9918).
Additional Product Information
CAP-D2 is a subunit of the heteropentameric condensin I complex. Condensin participates in the condensation of chromosomes during cell division by facilitating positive DNA supercoiling and knotting by topoisomerase I and II and proper segregation of sister chromatids. In the condensin complex CAP-D2 may be important for targeting condensin to mitotic chromosomes as well as metaphase alignment of sister chromatids.
Alternate Names
CAP-D2; chromosome condensation-related SMC-associated protein 1; chromosome-associated protein D2; CNAP1; condensin complex subunit 1; hCAP-D2; MCPH21; Non-SMC condensin I complex subunit D2; XCAP-D2 homolog
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.