Rabbit anti-CAPZB Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-734A maps to a region between residue 25 to 75 of human Capping Protein (Actin Filament) Muscle Z-Line, Beta using the numbering given in entry NP_001193469.1 (GeneID 832).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CAPZB immobilized on solid support.
The epitope recognized by A304-734A-T maps to a region between residue 25 to 75 of human Capping Protein (Actin Filament) Muscle Z-Line, Beta using the numbering given in entry NP_001193469.1 (GeneID 832).
Additional Product Information
CAPZB is the beta subunit of the barbed-end actin binding protein, which belongs to the F-actin capping protein family. The capping protein is a heterodimeric actin capping protein that blocks actin filament assembly and disassembly at the fast growing (barbed) filament ends and functions in regulating actin filament dynamics as well as in stabilizing actin filament lengths in muscle and nonmuscle cells [taken from NCBI Entrez Gene (Gene ID: 832)].
Alternate Names
CAPB; CAPPB; capping actin protein of muscle Z-line beta subunit; capping protein (actin filament) muscle Z-line, beta; CAP; ZcapZ beta; epididymis secretory sperm binding protein; F-actin-capping protein subunit beta
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.