Rabbit anti-CARM1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-421A maps to a region between residue 550 and the C-terminus (residue 585) of human Coactivator-Associated Arginine Methyltransferase 1 using the numbering given in Swiss-Prot entry Q86X55 (GeneID 10498).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CARM1 immobilized on solid support.
The epitope recognized by A300-421A-T maps to a region between residue 550 and the C-terminus (residue 585) of human Coactivator-Associated Arginine Methyltransferase 1 using the numbering given in Swiss-Prot entry Q86X55 (GeneID 10498).
Additional Product Information
Protein arginine methylation is a form of post-translational modification that is catalyzed by protein arginine methyltransferases (PRMTs). CARM1 (coactivator-associated arginine methyltransferase 1), also known as PRMT4, has been shown to interact with and methylate RNA splicing factors such as CA150 that link transcription to splicing. In this context, CARM1 may be involved in spinal muscular atrophy pathogenesis. CARM1 has also been found to be a coactivator of the NFKappaB transcription factor and the androgen receptor.
Alternate Names
Coactivator-associated arginine methyltransferase 1; histone-arginine methyltransferase CARM1; PRMT4; protein arginine N-methyltransferase 4
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.