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Rabbit anti-CARS Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
CARS
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 698 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to CARS immobilized on solid support.

The epitope recognized by A302-409A maps to a region between residue 698 and 748 of human cysteinyl-tRNA synthetase using the numbering given in entry NP_001742.1 (GeneID 833).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to CARS immobilized on solid support.

The epitope recognized by A302-409A-T maps to a region between residue 698 and 748 of human cysteinyl-tRNA synthetase using the numbering given in entry NP_001742.1 (GeneID 833).
Country of Origin
USA

Additional Product Information

Cysteinyl-tRNA synthetase (CARS) catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. The CARS gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. [taken from NCBI Entrez Gene (GeneID:833)].

833
CARS
cysteinyl-tRNA synthetase
P49589
Cysteine--tRNA ligase, cytoplasmic

Alternate Names

CARS1; CYSRS; cysteine translase; cysteine tRNA ligase 1, cytoplasmic; cysteine--tRNA ligase, cytoplasmic; Cysteinyl-tRNA synthetase; MGC:11246

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.