Rabbit anti-CARS Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-409A maps to a region between residue 698 and 748 of human cysteinyl-tRNA synthetase using the numbering given in entry NP_001742.1 (GeneID 833).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CARS immobilized on solid support.
The epitope recognized by A302-409A-T maps to a region between residue 698 and 748 of human cysteinyl-tRNA synthetase using the numbering given in entry NP_001742.1 (GeneID 833).
Additional Product Information
Cysteinyl-tRNA synthetase (CARS) catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. The CARS gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. [taken from NCBI Entrez Gene (GeneID:833)].
Alternate Names
CARS1; CYSRS; cysteine translase; cysteine tRNA ligase 1, cytoplasmic; cysteine--tRNA ligase, cytoplasmic; Cysteinyl-tRNA synthetase; MGC:11246
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.