Rabbit anti-CASC3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-472A maps to a region between residue 653 and 703 of human cancer susceptibility candidate 3 using the numbering given in entry NP_031385.2 (GeneID 22794).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CASC3 immobilized on solid support.
The epitope recognized by A302-472A-T maps to a region between residue 653 and 703 of human cancer susceptibility candidate 3 using the numbering given in entry NP_031385.2 (GeneID 22794).
Additional Product Information
Cancer susceptibility candidate gene 3 protein (CASC3) is a core component of the exon junction complex (EJC), a protein complex that is deposited on spliced mRNAs at exon-exon junctions and functions in nonsense-mediated mRNA decay (NMD). The encoded protein binds RNA and interacts with two other EJC core components. CASC3 is predominantly located in the cytoplasm, but shuttles into the nucleus where it localizes to nuclear speckles [taken from NCBI Entrez Gene (GeneID: 22794)].
Alternate Names
barents; ZBT; Zcancer susceptibility 3; cancer susceptibility candidate 3; cancer susceptibility candidate gene 3 protein; metastatic lymph node 51; metastatic lymph node gene 51 protein; MLN 51; MLN51; protein barents; Zprotein CASC3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.