Rabbit anti-CCDC131 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-455A maps to a region between residue 300 and 350 of human coiled-coil domain containing 131 using the numbering given in entry NP_659419.3 (GeneID 196441).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CCDC131 immobilized on solid support.
The epitope recognized by A301-455A-T maps to a region between residue 300 and 350 of human coiled-coil domain containing 131 using the numbering given in entry NP_659419.3 (GeneID 196441).
Additional Product Information
CCDC131 (coiled-coil domain containing protein 131) is an uncharacterized proline/serine rich coiled-coil protein that bears 6 HAT repeats- and 5 TPR repeats. Coiled-coil proteins exhibit tertiary structures made up of a number of highly organized alpha helices wound around each other to mediate oligomerization. HAT (half a TPR) repeats form amphipathic alpha-helices and are similar to TPR repeats which are 34 amino acid repeat motifs which form alpha helical structures.
Alternate Names
CCDC131; coiled-coil domain containing 131; coiled-coil domain-containing protein 131; CSRC2; proline/serine-rich coiled-coil 2; proline/serine-rich coiled-coil protein 2; PSRC2; zinc finger C3H1 domain-containing protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.