Rabbit anti-CCT2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-478A maps to a region between residue 325 and 375 of human Chaperonin Containing TCP1, Subunit 2 using the numbering given in entry NP_006422.1 (GeneID 10576).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CCT2 immobilized on solid support.
The epitope recognized by A303-478A-T maps to a region between residue 325 and 375 of human Chaperonin Containing TCP1, Subunit 2 using the numbering given in entry NP_006422.1 (GeneID 10576).
Additional Product Information
Chaperonin containing TCP1, subunit 2 (beta) (CCT2) is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin [taken from NCBI Entrez Gene (Gene ID: 10576)].
Alternate Names
99D8.1; CCTB; CCT-beta; chaperonin containing t-complex polypeptide 1, beta subunit; chaperonin containing t-complex polypeptide 1, subunit 2; chaperonin containing TCP1, subunit 2 (beta); epididymis secretory sperm binding protein Li 100n; HEL-S-100n; PRO1633; T-complex protein 1 subunit beta; T-complex protein 1, beta subunit; TCP-1-beta
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.