Rabbit anti-CCT4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-726A maps to a region between residue 489 to 539 of human Chaperonin Containing Tcp1, Subunit 4 (Delta) using the numbering given in entry NP_006421.2 (GeneID 10575).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CCT4 immobilized on solid support.
The epitope recognized by A304-726A-T maps to a region between residue 489 to 539 of human Chaperonin Containing Tcp1, Subunit 4 (Delta) using the numbering given in entry NP_006421.2 (GeneID 10575).
Additional Product Information
Chaperonin containing TCP1 subunit 4 (delta) or CCT4 is a molecular chaperone. It assists with the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, it may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. It is also known to play a role, in vitro, in the folding of actin and tubulin [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P50991].
Alternate Names
Cctd; CCT-DELTA; chaperonin containing t-complex polypeptide 1, delta subunit; chaperonin containing TCP1, subunit 4 (delta); SRB; stimulator of TAR RNA-binding; T-complex protein 1 subunit delta; TCP-1-delta
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.