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Rabbit anti-CD247/CD3Z Recombinant Monoclonal Antibody [BL-336-1B2]

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
CD247/CD3Z
Reactivity:
Human
Platforms:
COMET™, Simple Western
Host:
Rabbit
Clonality:
Recombinant Monoclonal
Clone:
BL-336-1B2
Format:
Whole IgG
Immunogen:
Between 114 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Purified
100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

$127.00 $794.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Mouse, Sheep, Pig
Antigen Species
Human
Concentration
100 µg/ml 1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Request Formulation Change
Production Procedures
Recombinant antibody was purified from cell culture supernatant.

Immunogen was a synthetic peptide representing a region between residue 150 and the C-terminus (residue 164) of human T-cell surface glycoprotein CD3 zeta chain using the numbering given in entry NP_932170.1 (GeneID 919).
Recombinant antibody was purified from cell culture supernatant.

Immunogen was a synthetic peptide representing a region between residue 150 and the C-terminus (residue 164) of human T-cell surface glycoprotein CD3 zeta chain using the numbering given in entry NP_932170.1 (GeneID 919).
Country of Origin
USA

Additional Product Information

T-cell surface glycoprotein CD3 zeta chain is encoded by the gene CD247, also known as CD3Z or CD3 zeta. CD247/CD3 zeta, together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response [taken from NCBI Entrez Gene (Gene ID: 919)].

919
CD247
CD247 molecule
P20963
T-cell surface glycoprotein CD3 zeta chain

Alternate Names

CD antigen CD247; CD247 antigen, zeta subunit; CD3H; CD3Q; CD3; ZCD3Z antigen, zeta polypeptide (TiT3 complex); CD3-ZETA; CD3zeta chain; IMD25; T3; ZT-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 zeta chain; T-cell surface glycoprotein CD3 zeta chain; TCR zeta chain; TCRZ

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.