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Rabbit anti-CD5 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
CD5
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 400 and 450
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Mouse, Bovine, Rat
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to CD5 immobilized on solid support.

The epitope recognized by A304-346A-T maps to a region between residue 375 to 425 of human CD5 Molecule using the numbering given in entry NP_055022.2 (GeneID 921).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to CD5 immobilized on solid support.

The epitope recognized by A304-346A maps to a region between residue 375 to 425 of human CD5 Molecule using the numbering given in entry NP_055022.2 (GeneID 921).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

CD5 may act as a receptor in regulating T-cell proliferation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P06127].

921
CD5
CD5 molecule
P06127
T-cell surface glycoprotein CD5

Alternate Names

CD antigen CD5; CD5 antigen (p56-62); epididymis secretory sperm binding protein; LEU1; lymphocyte antigen T1/Leu-1; T1; T-cell surface glycoprotein CD5

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.