Rabbit anti-CDC16/APC6 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-165A maps to a region between residue 175 and 225 of human cell division cycle 16 (anaphase-promoting complex subunit 6) using the numbering given in entry NP_003894.3 (GeneID 8881).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CDC16/APC6 immobilized on solid support.
The epitope recognized by A301-165A-T maps to a region between residue 175 and 225 of human cell division cycle 16 (anaphase-promoting complex subunit 6) using the numbering given in entry NP_003894.3 (GeneID 8881).
Additional Product Information
CDC15/APC6 is a component of the anaphase-promoting complex (APC). The APC complex functions as an ubiquitin ligase that controls cell cycle progression and promotes the transition from metaphase to anaphase. The APC complex is composed of at least 11 APC components. CDC15/APC6 amplification is associated with hepatocellular carcinoma.
Alternate Names
ANAPC6; Anaphase-promoting complex subunit 6; anaphase-promoting complex, subunit 6; APC6; CDC16 homolog; CDC16Hs; cell division cycle 16 homolog; cell division cycle protein 16 homolog; CUT9; cyclosome subunit 6
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.