Rabbit anti-CDC2L5 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-458A maps to a region between residue 1125 and 1175 of human cell division cycle 2-like 5 using the numbering given in entry NP_003709.3 (GeneID 8621).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CDC2L5 immobilized on solid support.
The epitope recognized by A301-458A-T maps to a region between residue 1125 and 1175 of human cell division cycle 2-like 5 using the numbering given in entry NP_003709.3 (GeneID 8621).
Additional Product Information
CDC2-like kinase 5 (CDC2L5) is a member of the cyclin-dependent serine/threonine protein kinase family that plays a central role in cell cycle control. The CDC2L5 cDNA was first identified as CHED (cholinesterase-related cell-division controller) and cloned serendipitously in a screen using a cholinesterase-specific oligonucleotide probe in search of a link between cholinergic signaling and hematopoietic cell division control. Recently, the understanding of the physiological function of CDC2L5 has become clearer. Due to its localization to speckles in the nucleoplasm and its interaction with the splicing protein, ASF/SF2-associated protein p32, it was suggested to play a role in mRNA processing. Further studies showed that overexpression of CDC2L5 altered mRNA splicing patterns, and that CDC2L5 associated with L-type cyclins known to be involved in alternative splicing. These results have led to the conclusion that CDC2L5 is part of a subfamily of cyclin dependent kinases that play a role in the regulation of alternative splicing.
Alternate Names
CDC2L; CDC2L5; CDC2-related protein kinase 5; cell division cycle 2-like protein kinase 5; cell division protein kinase 13; CHDFIDD; CHED; cholinesterase-related cell division controller; cyclin-dependent kinase 13; hCDK13
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.