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Rabbit anti-CDK2 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
CDK2
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 248 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (50+ slides)

10 µl (5+ slides)

$131.00 $492.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
Golden Hamster, Horse, Goat, Sheep, Dog, Pig, Rabbit, Bovine, Monkey, Rat
Antigen Species
Human
Concentration
250 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to CDK2 immobilized on solid support.

The epitope recognized by IHC-00374 maps to a region between residue 248 and 298 of human cyclin-dependent kinase 2 using the numbering given in entry NP_001789.2 (GeneID 1017).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to CDK2 immobilized on solid support.

The epitope recognized by IHC-00374-T maps to a region between residue 248 and 298 of human cyclin-dependent kinase 2 using the numbering given in entry NP_001789.2 (GeneID 1017).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

Cyclin-dependent kinase 2 (CDK2) is a member of the CDK family of protein kinases that regulate cell cycle progression. CDK2 associates with cyclin E or cyclin A to drive the cell cycle through G1- and S-phase. Substrates of CDK2 include pRb, p53, p27, E2F, Brca1, CDK7, B-Myb, PP1 alpha, RNA Pol II CTD, axin, p220 NPAT, ribonucleotide reductase M2, Myo-D, MARCKS, HsCdc6, and CP110.

1017
CDK2
cyclin dependent kinase 2
P24941
Cyclin-dependent kinase 2

Alternate Names

cdc2-related protein kinase; CDKN2; cell division protein kinase 2; cyclin-dependent kinase 2; p33 protein kinase; p33(CDK2)

Applications

Epitope exposure is recommended.

Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.