Rabbit anti-CENP-I Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-374A maps to a region between residue 50 and 100 of human Centromere Protein I using the numbering given in entry NP_006724.2 (GeneID 2491).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CENP-I immobilized on solid support.
The epitope recognized by A303-374A-T maps to a region between residue 50 and 100 of human Centromere Protein I using the numbering given in entry NP_006724.2 (GeneID 2491).
Additional Product Information
Centromere protein I (CENP-I) is a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. CENP-I may be involved in incorporation of newly synthesized CENP-A into centromeres via its interaction with the CENPA-NAC complex. CENP-I is required for the localization of CENP-F, MAD1L1 and MAD2 (MAD2L1 or MAD2L2) to kinetochores. CENP-A is involved in the response of gonadal tissues to follicle-stimulating hormone [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q92674].
Alternate Names
CENP-I; centromere protein I; follicle-stimulating hormone primary response protein; FSH primary response 1; FSH primary response protein 1; FSHPRH1; interphase centromere complex protein 19; leucine-rich primary response protein 1; LRPR1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.