Rabbit anti-CHAMP1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-217A maps to a region between residue 762 to 812 of human Chromosome Alignment Maintaining Phosphoprotein 1 using the numbering given in entry NP_115812.1 (GeneID 283489).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CHAMP1 immobilized on solid support.
The epitope recognized by A304-217A-T maps to a region between residue 762 to 812 of human Chromosome Alignment Maintaining Phosphoprotein 1 using the numbering given in entry NP_115812.1 (GeneID 283489).
Additional Product Information
Chromosome alignment-maintaining phosphoprotein (CHAMP1) is required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. It is involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. CHAMP1 may recruit CENPE and CENPF to the kinetochore [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96JM3].
Alternate Names
C13orf8; CAMP; CHAMP; chromosome alignment-maintaining phosphoprotein 1; MRD40; zinc finger protein 828; ZNF828
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.