Rabbit anti-CHCHD3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-497A maps to a region between residue 177 to 227 of human Coiled-coil-helix-coiled-coil-helix domain-containing protein 3, mitochondrial using the numbering given in entry Q9NX63.1 (GeneID 54927).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CHCHD3 immobilized on solid support.
The epitope recognized by A305-497A-T maps to a region between residue 177 to 227 of human Coiled-coil-helix-coiled-coil-helix domain-containing protein 3, mitochondrial using the numbering given in entry Q9NX63.1 (GeneID 54927).
Additional Product Information
CCoiled-coil-helix-coiled-coil-helix domain-containing protein 3 (CHCHD3) is an inner mitochondrial membrane scaffold protein. Absence of CHCHD3 affects the structural integrity of mitochondrial cristae and leads to reductions in ATP production, cell growth, and oxygen consumption. CHCHD3 is part of the mitochondrial contact site and cristae organizing system (MICOS) [taken from NCBI Entrez Gene (Gene ID: 54927)].
Alternate Names
Coiled-coil-helix-coiled-coil-helix domain-containing protein 3; coiled-coil-helix-coiled-coil-helix domain-containing protein 3, mitochondrial; Mic19; MICOS complex subunit MIC19; MINOS3; mitochondrial inner membrane organizing system 3; PPP1R22; protein phosphatase 1, regulatory subunit 22
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.