Rabbit anti-CHD3 IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00247 maps to a region between residue 1950 and 2000 of human chromodomain helicase DNA binding protein 3 using the numbering given in entry NP_001005273.1 (GeneID 1107).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CHD3 immobilized on solid support.
The epitope recognized by IHC-00247-T maps to a region between residue 1950 and 2000 of human chromodomain helicase DNA binding protein 3 using the numbering given in entry NP_001005273.1 (GeneID 1107).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
CHD3 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and functional domains: a chromodomain (chromatin organization modifier), an SNF2-like helicase/ATPase domain, and a C-terminal DNA-binding domain. CHD3 is a key component of the NuRD (nucleosomal remodeling) complex and directly interacts with histone deacetylases 1 and 2, ATR, and TRIM 27. Studies in several species suggest a role for CHD3 in the repression of developmental genes.
Alternate Names
ATP-dependent helicase CHD3; CHD-3; chromodomain-helicase-DNA-binding protein 3; hZFH; mi-2 autoantigen 240 kDa protein; Mi-2a; Mi2-ALPHA; ZFH; zinc finger helicase; zinc-finger helicase (Snf2-like)
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.