Rabbit anti-CHD8 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-224A maps to a region between residue 325 and 350 of human chromodomain helicase DNA binding protein 8 using the numbering given in entry NP_065971.2 (GeneID 57680).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CHD8 immobilized on solid support.
The epitope recognized by A301-224A-T maps to a region between residue 325 and 350 of human chromodomain helicase DNA binding protein 8 using the numbering given in entry NP_065971.2 (GeneID 57680).
Additional Product Information
CHD8 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and functional domains: a chromodomain (chromatin organization modifier), an SNF2-like helicase/ATPase domain, and a C-terminal DNA-binding domain. CHD8 has been reported to interact with duplin, a protein involved in the wnt signaling pathway, and CTCF, a protein involved in heterochromatin formation.
Alternate Names
ATP-dependent helicase CHD8; AUTS18; axis duplication inhibitor; CHD-8; chromodomain-helicase-DNA-binding protein 8; duplin; helicase with SNF2 domain 1; HELSNF1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.