Rabbit anti-CIR1 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Corepressor interacting with RBPJ (CIR1) may modulate splice site selection during alternative splicing of pre-mRNAs (by similarity). CIR1 regulates transcription and acts as a corepressor for RBPJ. Recruits RBPJ to the Sin3-histone deacetylase complex (HDAC) and is required for RBPJ-mediated repression of transcription [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q86X95].
Alternate Names
CBF1-interacting corepressor; CIR; corepressor interacting with RBPJ 1; recepin
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.