Rabbit anti-CIZ1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-497A maps to a region between residue 848 and 898 of human CDKN1A interacting zinc finger protein 1 using the numbering given in entry NP_036259.2 (GeneID 25792).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CIZ1 immobilized on solid support.
The epitope recognized by A301-497A-T maps to a region between residue 848 and 898 of human CDKN1A interacting zinc finger protein 1 using the numbering given in entry NP_036259.2 (GeneID 25792).
Additional Product Information
CIZ1 (Cip1 interacting zinc-finger protein) was identified as a protein that associates with the cell-cycle inhibiting protein, p21 (Cip1/Waf1). CIZ1 is a member of the matrin 3 protein family and has been shown to bind DNA consensus sequences and co-localize with PCNA during replication. In breast cancer cells, CIZ1 was shown to associate with Cdk2 and dynein light chain 1 (DLC1), a cytoskeletal signaling protein involved in cell cycle regulation and tumorigenesis. Studies of the role of CIZ1 in breast tumorigenesis revealed that CIZ1 is an estrogen-responsive gene that functions as a coactivator of the estrogen receptor. Targeted depletion of CIZ1 inhibits entry into S-phase. This finding along with evidence that CIZ1 interacts with multiple cell cycle regulatory proteins and localizes to DNA replication foci, indicates that CIZ1 plays a critical role in the regulation of DNA replication.
Alternate Names
CDKN1A-interacting zinc finger protein 1; cip1-interacting zinc finger protein; LSFR1; NP94; nuclear protein NP94; zinc finger protein 356; ZNF356
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.