Rabbit anti-CKAP4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-256A maps to a region between residue 375 and 425 of human cytoskeleton-associated protein 4 using the numbering given in entry NP_006816.2 (GeneID 10970).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CKAP4 immobilized on solid support.
The epitope recognized by A302-256A-T maps to a region between residue 375 and 425 of human cytoskeleton-associated protein 4 using the numbering given in entry NP_006816.2 (GeneID 10970).
Additional Product Information
Cytoskeleton-associated protein 4 (CKAP4) is also known as p63 and cytoskeleton-linking membrane protein 63kDa (CLIMP-63). It was first described as a palmitoylated single-pass transmembrane receptor that localized to the rough endoplasmic reticulum as well as the cell membrane. CKAP4 has been shown to help anchor endoplasmic reticulum to microtubules.
Alternate Names
63 kDa membrane protein; 63-kDa cytoskeleton-linking membrane protein; CLIMP-63; cytoskeleton-associated protein 4; ERGIC-63; p63; transmembrane protein (63kD), endoplasmic reticulum/Golgi intermediate compartment; type-II transmembrane protein p63
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.