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Rabbit anti-CLIP170 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
CLIP170
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1350 and 1400
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Mouse
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to CLIP170 immobilized on solid support.

The epitope recognized by A303-344A maps to a region between residue 1338 and 1388 of human Cytoplasmic Linker Protein 170 Alpha-2 using the numbering given in entry AAI26306.1 (GeneID 6249).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to CLIP170 immobilized on solid support.

The epitope recognized by A303-344A-T maps to a region between residue 1338 and 1388 of human Cytoplasmic Linker Protein 170 Alpha-2 using the numbering given in entry AAI26306.1 (GeneID 6249).
Country of Origin
USA

Additional Product Information

CLIP-170 (cytoplasmic linker protein 170) is a microtubule plus end binding protein (+TIPs) that plays a role in multimerizing tubulin and the formation of kinetochore-microtubule attachments.

6249
CLIP1
CAP-Gly domain containing linker protein 1
P30622
CAP-Gly domain-containing linker protein 1

Alternate Names

CAP-Gly domain-containing linker protein 1; CLIP; CLIP170; CLIP-170; CYLN1; cytoplasmic linker protein 1; cytoplasmic linker protein 170 alpha-2; cytoplasmic linker protein CLIP-170; Reed-Sternberg intermediate filament-associated protein; Restin; restin (Reed-Steinberg cell-expressed intermediate filament-associated protein); RSN

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.