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Rabbit anti-CNOT3 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
CNOT3
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 525 and 575
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (50+ slides)

10 µl (5+ slides)

$121.00 $459.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Dog, Monkey
Antigen Species
Human
Concentration
250 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to CNOT3 immobilized on solid support.

The epitope recognized by IHC-00623 maps to a region between residue 525 and 575 of human CCR4-NOT transcription complex, subunit 3 using the numbering given in entry NP_055331.1 (GeneID 4849).

Immunoglobulin concentration was determined using Beer���s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to CNOT3 immobilized on solid support.

The epitope recognized by IHC-00623-T maps to a region between residue 525 and 575 of human CCR4-NOT transcription complex, subunit 3 using the numbering given in entry NP_055331.1 (GeneID 4849).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

CNOT3 (CCR4-NOT transcription complex subunit 3) is a component of the CCR4-NOT core complex that functions as a general transcriptional regulator. In a genome-wide siRNA screen for genes essential for mouse embryonic stem cell self-renewal, CNOT3 was identified and found to co-occupy gene promoters with c-Myc and Zfx.

4849
CNOT3
CCR4-NOT transcription complex subunit 3
O75175
CCR4-NOT transcription complex subunit 3

Alternate Names

CCR4-associated factor 3; CCR4-NOT transcription complex subunit 3; LENG2; leukocyte receptor cluster member 2; NOT3; NOT3 (negative regulator of transcription 3, yeast) homolog; NOT3H

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.