Rabbit anti-CoAA Antibody, Affinity Purified
Product Details
Specifications
The epitope recognized by A300-845A maps to a region between residue 550 and 600 of human CoActivator Activator (RNA Binding Motif 14) using the numbering given in entry NP_006319.1 (GeneID 10432).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CoAA immobilized on solid support.
The epitope recognized by A300-845A-T maps to a region between residue 550 and 600 of human CoActivator Activator (RNA Binding Motif 14) using the numbering given in entry NP_006319.1 (GeneID 10432).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
CoAA (coactivator activator) is also known as RBM14 (RNA binding motif protein 14) because of the presence of two RNA recognition motifs (RRMs) in its N-terminus. CoAA has been observed to mediate the coupled processes of transcription and splicing in a promoter-specific manner.
Alternate Names
COAA; paraspeckle protein 2; PSP2; RNA-binding motif protein 14; RNA-binding protein 14; RRM-containing coactivator activator/modulator; SIP; synaptotagmin-interacting protein; SYT-interacting protein; SYTIP1; TMEM137; transmembrane protein 137
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.