Rabbit anti-CPSF30 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-584A maps to a region between residue 220 and 245 of human cleavage and polyadenylation specific factor 4, 30kD subunit using the numbering given in entry NP_006684.1 (GeneID 10898).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CPSF30 immobilized on solid support.
The epitope recognized by A301-584A-T maps to a region between residue 220 and 245 of human cleavage and polyadenylation specific factor 4, 30kD subunit using the numbering given in entry NP_006684.1 (GeneID 10898).
Additional Product Information
CPSF30/CPSF4 (cleavage and polyadenylation specific factor 4, 30 kDa subunit) is a subunit of the cleavage and polyadenylation specificity factor (CPSF) involved in mammalian mRNA 3’-end processing. CPSF, along with CstF (cleavage and stimulation factor), is critical for the recognition of cis-acting sequence elements on the pre-mRNA that dictate the site of cleavage and subsequent polyadenylation. CPSF exists as a complex of four proteins that includes CPSF160/CPSF1, CPSF100/CPSF2, CPSF73/CPSF3, and CPSF30/CPSF4. Studies of the Arabidopsis CPSF30/CPSF4 ortholog have revealed that CPSF30/CPSF4 may function as a processing endonuclease and interacts with Fip1, an integral subunit of CPSF.
Alternate Names
cleavage and polyadenylation specific factor 4, 30kDa; cleavage and polyadenylation specificity factor 30 kDa subunit; cleavage and polyadenylation specificity factor subunit 4; CPSF 30 kDa subunit; CPSF30; NAR; NEB1; NEB-1; no arches homolog; no arches-like zinc finger protein; NS1 effector domain-binding protein 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.