Rabbit anti-CPSF68 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-356A maps to a region between residue 1 and 50 of human cleavage and polyadenylation specific factor 6, 68kDa using the numbering given in entry NP_008938.1 (GeneID 11052).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CPSF68 immobilized on solid support.
The epitope recognized by A301-356A-T maps to a region between residue 1 and 50 of human cleavage and polyadenylation specific factor 6, 68kDa using the numbering given in entry NP_008938.1 (GeneID 11052).
Additional Product Information
Cleavage and polyadenylation specific factor 68 (CPSF68) is the 68 kDa subunit of the cleavage factor Im complex (CFIm). The CFIm is a heterodimer composed of a 25 kDa (CPSF5) subunit and either the 59 kDa (CPSF7) or 68 kDa (CPSF6) subunit and is essential to the first step of pre-mRNA 3’-end processing. CPSF68 bears an N-terminal RNA- recognition motif (RRM) that is necessary for binding CPS5.
Alternate Names
CFIM; CFIM68; CFIM72; cleavage and polyadenylation specific factor 6, 68kDa; cleavage and polyadenylation specificity factor 68 kDa subunit; cleavage and polyadenylation specificity factor subunit 6; cleavage factor Im complex 68 kDa subunit; CPSF 68 kDa subunit; HPBRII-4; HPBRII-7; pre-mRNA cleavage factor I, 68kD subunit; pre-mRNA cleavage factor Im (68kD); pre-mRNA cleavage factor Im 68 kDa subunit; protein HPBRII-4/7
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.