Rabbit anti-CRSP2/DRIP150 Antibody, Affinity Purified
Product Details
Specifications
The epitope recognized by A301-044A-T maps to a region between residue 1 and 50 of human cofactor required for Sp1 transcriptional activation, subunit 2 (vitamin D receptor-interacting protein complex component DRIP150 ) using the numbering given in entry NP_004220.2 (GeneID 9282). Antibody was affinity purified using an epitope specific to CRSP2/DRIP150 immobilized on solid support.
The epitope recognized by A301-044A maps to a region between residue 1 and 50 of human cofactor required for Sp1 transcriptional activation, subunit 2 (vitamin D receptor-interacting protein complex component DRIP150 ) using the numbering given in entry NP_004220.2 (GeneID 9282).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
CRSP2 is a subunit of the CRSP (cofactor required for SP1 activation) complex required for efficient transcriptional activation by the SP1 transcription factor. It has also been identified as a component of the mediator complex that bridges nuclear hormone receptors to RNA polymerase II.
Alternate Names
activator-recruited cofactor 150 kDa component; ARC150; Cofactor required for Sp1 transcriptional activation subunit 2; CRSP complex subunit 2; CRSP150; CRSP2; CSRP; CXorf4; DRIP150; EXLM1; hRGR1; human homolog of yeast RGR1; Mediator complex subunit 14; mediator of RNA polymerase II transcription subunit 14; RGR1; RGR1 homolog; thyroid hormone receptor-associated protein complex component TRAP170; Transcriptional coactivator CRSP150; TRAP170; vitamin D receptor-interacting protein complex component DRIP150; vitamin D3 receptor-interacting protein complex 150 kDa component
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.