Rabbit anti-CtIP Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-488A maps to a region between residue 850 and the C-terminus (residue 897) of human CtBP Interacting Protein (Retinoblastoma Binding Protein 8) using the numbering given in entry NP_002885.1 (GeneID 5932).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CtIP (RBBP8) immobilized on solid support.
The epitope recognized by A300-488A-T maps to a region between residue 850 and the C-terminus (residue 897) of human CtBP Interacting Protein (Retinoblastoma Binding Protein 8) using the numbering given in entry NP_002885.1 (GeneID 5932).
Additional Product Information
CtIP (CTBP interacting protein) was first identified as a protein that interacted with CTBP (C-terminus-binding protein of adenovirus E1A). CtIP has also been identified as a protein that interacts with the tumor suppressors pRb and BRCA1 and has been implicated as a tumor suppressor itself.
Alternate Names
COM1; CTBP-interacting protein; CTIP; DNA endonuclease RBBP8; JWDS; RBBP-8; retinoblastoma binding protein 8; Retinoblastoma-binding protein 8; Retinoblastoma-interacting protein and myosin-like; RIM; SAE2; SCKL2; sporulation in the absence of SPO11 protein 2 homolog
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.