Rabbit anti-Cul2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-475A-T maps to a region between residue 150 and 200 of human cullin 2 using the numbering given in entry NP_003582.2 (GeneID 8453).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Cul2 immobilized on solid support.
The epitope recognized by A302-475A maps to a region between residue 150 and 200 of human cullin 2 using the numbering given in entry NP_003582.2 (GeneID 8453).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Cullin-2 (Cul2) is a core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. Cul2 may serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q13617].
Alternate Names
CUL-2; cullin-2; testis secretory sperm-binding protein Li 238E
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.