Rabbit anti-Cul5 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-173A maps to a region between residue 1 and 50 of human cullin-5 using the numbering given in entry NP_003469.2 (GeneID 8065).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Cullin-5 (Cul-5) is a core component of multiple E3 ubiquitin ligase complexes.
Alternate Names
CUL-5; cullin-5; Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1); VACM1; VACM-1; vasopressin-activated calcium-mobilizing receptor 1; Vasopressin-activated calcium-mobilizing receptor-1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.