Rabbit anti-Cytokeratin 5/6 Recombinant Monoclonal Antibody [BLR340M]
Product Details
Specifications
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Immunogen was a peptide representing a region between residue 540 and the C-terminus (residue 590) of human Cytoskeletal Keratin 5 using the numbering given in entry NP_000415.2 (Gene ID 3852).
Additional Product Information
Cytokeratins 5 and 6 are type II cytokeratins and consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. In normal tissue, Cyokeratin 5 is specifically expressed in the basal layer of the epidermis with family member KRT14 while Cytokeratin 6 is expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. Cytokeratins 5 and 6 are often referred to together as "CK5/6, and are commonly expressed in various cancers, particularly those originating from epithelial tissues, and their presence is often used as a marker to identify squamous cell carcinomas or tumors with a basal cell-like phenotype [taken from NCBI Entrez Gene (Gene ID: 3852 and 3853)].
Alternate Names
58 kda cytokeratin;CK5;CK-5;cytokeratin-5;DDD;DDD1;EBS2;epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types;K5;keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types);keratin 5, type II;keratin, type II cytoskeletal 5;Keratin-5;KRT5A;type-II keratin Kb5;allergen Hom s 5;CK6A;CK-6A;CK6C;CK-6C;CK6D;CK-6D;CK-6E;cytokeratin 6A;cytokeratin 6C;cytokeratin 6D;Cytokeratin-6A;Cytokeratin-6D;K6A;K6C;K6D;keratin 6A, type II;keratin, epidermal type II, K6A;keratin, type II cytoskeletal 6A;keratin, type II cytoskeletal 6C;Keratin-6A;KRT6C;KRT6D;PC3;type-II keratin Kb6
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).