Rabbit anti-Daple Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-951A-T maps to a region between residue 1450 and 1500 of human Dvl-Associating Protein with a High Frequency of Leucine Residues using the numbering given in entry NP_001073883.2 (GeneID 440193).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Daple immobilized on solid support.
The epitope recognized by A302-951A maps to a region between residue 1450 and 1500 of human Dvl-Associating Protein with a High Frequency of Leucine Residues using the numbering given in entry NP_001073883.2 (GeneID 440193).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Daple (Dvl-associating protein with a high frequency of leucine residues) is a negative regulator of the canonical Wnt signaling pathway and acts downstream of DVL to inhibit CTNNB1/Beta-catenin stabilization [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9P219].
Alternate Names
Coiled-coil domain-containing protein 88C; DAPLE; Dvl-associating protein with a high frequency of leucine residues; hDaple; HKRP2; hook-related protein 2; HYC1; KIAA1509; protein Daple; SCA40; spinocerebellar ataxia 40
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.