Rabbit anti-Dcp2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-597A maps to a region between residue 370 and 420 of human mRNA-decapping enzyme 2 using the numbering given in entry NP_689837.2 (GeneID 167227).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Dcp2 immobilized on solid support.
The epitope recognized by A302-597A-T maps to a region between residue 370 and 420 of human mRNA-decapping enzyme 2 using the numbering given in entry NP_689837.2 (GeneID 167227).
Additional Product Information
mRNA-decapping enzyme 2 (Dcp2) is required for both normal mRNA turnover and nonsense-mediated mRNA decay. Dcp2 plays a role in replication-dependent histone mRNA degradation. It removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8IU60].
Alternate Names
DCP2 decapping enzyme homolog; hDpc; m7GpppN-mRNA hydrolase; mRNA-decapping enzyme 2; Nucleoside diphosphate-linked moiety X motif 20; nudix (nucleoside diphosphate linked moiety X)-type motif 20; Nudix motif 20; NUDT20
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.