Rabbit anti-DCPS Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-426A-T maps to a region between residue 1 to 50 of human Scavenger mRNA-decapping enzyme DcpS using the numbering given in entry Q96C86.2 (GeneID 28960).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DCPS immobilized on solid support.
The epitope recognized by A305-426A maps to a region between residue 1 to 50 of human Scavenger mRNA-decapping enzyme DcpS using the numbering given in entry Q96C86.2 (GeneID 28960).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
DCPS is a decapping scavenger enzyme that catalyzes the cleavage of a residual cap structure following the degradation of mRNAs by the 3'->5' exosome-mediated mRNA decay pathway. DCPS Hydrolyzes cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG) with up to 10 nucleotide substrates (small capped oligoribonucleotides) and specifically releases 5'-phosphorylated RNA fragments and 7-methylguanosine monophosphate (m7GMP)[taken from NCBI Entrez Gene (Gene ID: 28960)].
Alternate Names
5'-(N(7)-methyl 5'-triphosphoguanosine)-mRNA diphosphatase; ARS; DCS1; DCS-1; decapping scavenger enzyme; epididymis secretory sperm binding protein; heat shock-like protein 1; HINT5; HINT-5; hint-related 7meGMP-directed hydrolase; histidine triad nucleotide-binding protein 5; histidine triad protein member 5; homolog of C. elegans 7meGMP-directed hydrolase dcs-1; HSL1; HSPC015; m7GpppX diphosphatase; mRNA decapping enzyme; scavenger mRNA-decapping enzyme DcpS
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.