Rabbit anti-DDX41 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-050A maps to a region between residue 572 and the C-terminus (residue 622) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 41 using the numbering given in entry NP_057306.2 (GeneID 51428).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DDX41 immobilized on solid support.
The epitope recognized by A301-050A-T maps to a region between residue 572 and the C-terminus (residue 622) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 41 using the numbering given in entry NP_057306.2 (GeneID 51428).
Additional Product Information
DDX41 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. The function of DDX41 has not been determined.
Alternate Names
ABS; DEAD (Asp-Glu-Ala-Asp) box polypeptide 41; DEAD box protein 41; DEAD box protein abstrakt homolog; DEAD-box protein abstrakt; MPLPF; probable ATP-dependent RNA helicase DDX41; putative RNA helicase
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.