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Rabbit anti-DDX43 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
DDX43
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 300 and 350
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to DDX43 immobilized on solid support.

The epitope recognized by A302-328A-T maps to a region between residue 275 and 325 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 43 using the numbering given in entry NP_061135.1 (GeneID 55510).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to DDX43 immobilized on solid support.

The epitope recognized by A302-328A maps to a region between residue 275 and 325 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 43 using the numbering given in entry NP_061135.1 (GeneID 55510).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

DDX43 is a DEAD box ATP-dependent RNA helicase. The DDX43 gene is also known as HAGE. It has been found to be expressed specifically in tumors.

55510
DDX43
DEAD-box helicase 43
Q9NXZ2
Probable ATP-dependent RNA helicase DDX43

Alternate Names

cancer/testis antigen 13; CT13; DEAD (Asp-Glu-Ala-Asp) box polypeptide 43; DEAD box protein 43; DEAD box protein HAGE; DEAD-box protein 43; HAGE; helical antigen; probable ATP-dependent RNA helicase DDX43

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.