Rabbit anti-DDX47 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-976A maps to a region between residue 1 and 50 of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 47 using the numbering given in entry NP_057439.2 (GeneID 51202).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DDX47 immobilized on solid support.
The epitope recognized by A302-976A-T maps to a region between residue 1 and 50 of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 47 using the numbering given in entry NP_057439.2 (GeneID 51202).
Additional Product Information
DDX47 (DEAD box polypeptide 47) is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX47 can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity.
Alternate Names
DEAD (Asp-Glu-Ala-Asp) box polypeptide 47; DEAD box polypeptide 47; DEAD box protein 47; E4-DBP; E4-DEAD box protein; HQ0256; MSTP162; probable ATP-dependent RNA helicase DDX47; RRP3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.