Rabbit anti-DDX55 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-027A maps to a region between residue 175 and 225 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 55 using the numbering given in entry NP_065987.1 (GeneID 57696).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DDX55 immobilized on solid support.
The epitope recognized by A303-027A-T maps to a region between residue 175 and 225 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 55 using the numbering given in entry NP_065987.1 (GeneID 57696).
Additional Product Information
DDX55 is a member of of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.DDX55 is a probable ATP-binding RNA helicase [taken from NCBI Entrez Gene (Gene ID: 57696)].
Alternate Names
ATP-dependent RNA helicase DDX55; DEAD (Asp-Glu-Ala-Asp) box polypeptide 55; DEAD box protein 55; epididymis secretory sperm binding protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.