Rabbit anti-DDX56 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-979A maps to a region between residue 497 and 547 of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 56 using the numbering given in entry NP_061955.1 (GeneID 54606).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DDX56 immobilized on solid support.
The epitope recognized by A302-979A-T maps to a region between residue 497 and 547 of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 56 using the numbering given in entry NP_061955.1 (GeneID 54606).
Additional Product Information
DDX56 (DEAD box polypeptide 56) is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX56 is propososed to be involved in ribosome biosynthesis and the assembly of the large (60S) ribosomal subunit.
Alternate Names
61-kd nucleolar helicase; ATP-dependent 61 kDa nucleolar RNA helicase; DDX21; DDX26; DEAD (Asp-Glu-Ala-Asp) box helicase 56; DEAD (Asp-Glu-Ala-Asp) box polypeptide 56; DEAD box protein 21; DEAD box protein 56; DEAD-box RNA helicase; NOH61; nucleolar helicase of 61 kDa; probable ATP-dependent RNA helicase DDX56; putative nucleolar RNA helicase
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.