Rabbit anti-DLG5 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-301A-T maps to a region between residue 800 and 850 of human discs, large homolog 5 using the numbering given in entry NP_004738.3 (GeneID 9231).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DLG5 immobilized on solid support.
The epitope recognized by A302-301A maps to a region between residue 800 and 850 of human discs, large homolog 5 using the numbering given in entry NP_004738.3 (GeneID 9231).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
DLG5 (discs large homolog 5) is a member of the family of discs large (DLG) homologs, a subset of the membrane-associated guanylate kinase (MAGUK) superfamily. The MAGUK proteins are composed of a catalytically inactive guanylate kinase domain, in addition to PDZ and SH3 domains, and are thought to function as scaffolding molecules at sites of cell-cell contact. DLG5 localizes to the plasma membrane and cytoplasm, and interacts with components of adherens junctions and the cytoskeleton. It is proposed to function in the transmission of extracellular signals to the cytoskeleton and in the maintenance of epithelial cell structure [taken from NCBI GeneID:9231].
Alternate Names
discs large protein LP-DLG; discs large protein P-dlg; discs, large homolog 5; disks large homolog 5; large type of P-DLG; LP-DLG; PDLG; P-DLG5; placenta and prostate DLG
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.