Rabbit anti-DOT1L Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
DOT1L is a histone methyltransferase that methylates nucleosomal histone H3 on lysine 79. In contrast to other lysine histone methyltransferase, DOT1L does not contain a SET domain, a domain shown to be necessary for methyltransferase activity.
Alternate Names
DOT1; DOT1 like histone H3K79 methyltransferase; DOT1-like histone methyltransferase; DOT1-like protein; DOT1-like, histone H3 methyltransferase; H3-K79-HMTase; histone H3-K79 methyltransferase; histone methyltransferase DOT1L; histone-lysine N-methyltransferase, H3 lysine-79 specific; KMT4; lysine N-methyltransferase 4
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.