Rabbit anti-DTX3L/BBAP Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-834A maps to a region between residue 700 and the C-terminus (residue 740) of human Deltex 3-Like Protein (B-Lymphoma- and BAL-Associated Protein) using the numbering given in entry NP_612144.1 (GeneID 151636).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DTX3L/BBAP immobilized on solid support.
The epitope recognized by A300-834A-T maps to a region between residue 700 and the C-terminus (residue 740) of human Deltex 3-Like Protein (B-Lymphoma- and BAL-Associated Protein) using the numbering given in entry NP_612144.1 (GeneID 151636).
Additional Product Information
The c-terminal of BBAP (B-lymphoma and BAL-binding protein) is highly homologous to the DTX family of proteins and like the DTX family of proteins, functions as an E3 ubiquitin ligase. BBAP associates with BAL1 a transcription factor linked to poor outcome in diffuse large B-cell lymphoma (DLBCL). BAL1 and BBAP expression has been shown to be coregulated, and BBAP has been found to regulate the subcellular localization of BAL1.
Alternate Names
BBAP; B-lymphoma- and BAL-associated protein; deltex 3 like, E3 ubiquitin ligase; deltex 3-like; E3 ubiquitin-protein ligase DTX3L; Protein deltex-3-like; rhysin2; rhysin-2; RING-type E3 ubiquitin transferase DTX3L; RNF143
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.