Rabbit anti-DYNC1H1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-720A maps to a region between residue 1250 to 1300 of human Dynein, Cytoplasmic 1, Heavy Chain 1 using the numbering given in entry NP_001367.2 (GeneID 1778).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DYNC1H1 immobilized on solid support.
The epitope recognized by A304-720A-T maps to a region between residue 1250 to 1300 of human Dynein, Cytoplasmic 1, Heavy Chain 1 using the numbering given in entry NP_001367.2 (GeneID 1778).
Additional Product Information
Dyneins are a group of microtubule-activated ATPases that function as molecular motors. They are divided into two subgroups of axonemal and cytoplasmic dyneins. The cytoplasmic dyneins function in intracellular motility, including retrograde axonal transport, protein sorting, organelle movement, and spindle dynamics. Molecules of conventional cytoplasmic dynein are comprised of 2 heavy chain polypeptides and a number of intermediate and light chains. DYNC1H1 is a member of the cytoplasmic dynein heavy chain family [taken from NCBI Entrez Gene (Gene ID: 1778)].
Alternate Names
CMT2O; cytoplasmic dynein 1 heavy chain 1; Cytoplasmic dynein heavy chain 1; DHC1; DHC1a; DNCH1; Dnchc1; DNCL; DNECL; DYHC; dynein heavy chain, cytosolic; dynein, cytoplasmic, heavy polypeptide 1; HL-3; p22; SMALED1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.