Rabbit anti-E2F1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-766A maps to a region between residue 387 and 437 of human E2F Transcription Factor 1 using the numbering given in entry NP_005216.1 (GeneID 1869).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to E2F1 immobilized on solid support.
The epitope recognized by A300-766A-T maps to a region between residue 387 and 437 of human E2F Transcription Factor 1 using the numbering given in entry NP_005216.1 (GeneID 1869).
Additional Product Information
E2F1 is a member of the E2F family of transcription factors. E2F is an activating member that binds DNA at the E2 recognition site in the promoters of genes involved in cell cycle regulation or DNA replication. E2F1 is regulated through its interaction with the pRb tumor suppressor.
Alternate Names
E2F-1; PBR3; PRB-binding protein E2F-1; RBAP1; RBAP-1; RBBP3; RBBP-3; RBP3; retinoblastoma-associated protein 1; retinoblastoma-binding protein 3; transcription factor E2F1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.