Rabbit anti-EEA1 IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00540 maps to a region between residue 100 and 150 of human early endosome antigen 1 using the numbering given in entry NP_003557.2 (GeneID 8411). Antibody was affinity purified using an epitope specific to EEA1 immobilized on solid support.
The epitope recognized by IHC-00540-T maps to a region between residue 100 and 150 of human early endosome antigen 1 using the numbering given in entry NP_003557.2 (GeneID 8411).
Additional Product Information
Early endosomal autoantigen 1 (EEA1) bears an FYVE (Fab1p-YOPB-Vps27p-EEA1) domain which is an approximately 80 amino acid double-zinc binding motif that binds to PtdIns(3)P (phosphatidylinositol 3-phosphate). The FYVE domain is found in proteins involved in intracellular signaling, membrane trafficking, and cytoskeletal rearrangement. EEA1 binds to early endosomal membranes that contain PtdIns(3)P and plays a role in the docking and fusion of early endosomes.
Alternate Names
early endosome antigen 1; early endosome antigen 1, 162kD; early endosome-associated protein; endosome-associated protein p162; MST105; MSTP105; ZFYVE2; zinc finger FYVE domain-containing protein 2
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.