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Rabbit anti-eEF2 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
eEF2
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 808 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (50+ slides)

10 µl (5+ slides)

$131.00 $492.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
Horse, Turkey, Dog, Chicken, Pig, X. laevis, Zebrafish, Bovine, Monkey, Rat
Antigen Species
Human
Concentration
100 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to eEF2 immobilized on solid support.

The epitope recognized by IHC-00330 maps to a region between residue 808 and 858 of human eukaryotic translation elongation factor 2 using the numbering given in entry NP_001952.1 (GeneID 1938).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to eEF2 immobilized on solid support.

The epitope recognized by IHC-00330-T maps to a region between residue 808 and 858 of human eukaryotic translation elongation factor 2 using the numbering given in entry NP_001952.1 (GeneID 1938).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

Eukaryotic translation elongation factor 2 (eEF2) is a non-ribosomal protein that mediates ribosomal translocation and catalyzes peptide elongation in the presence of GTP. eEF2 is a substrate of the eEF2 kinase. Phosphorylation of eEF2 in response to increased energy demand or reduction in supply inhibits eEF2 activity for the conservation of energy.

1938
EEF2
eukaryotic translation elongation factor 2
P13639
Elongation factor 2

Alternate Names

EEF-2; EF2; EF-2; elongation factor 2; epididymis secretory sperm binding protein; polypeptidyl-tRNA translocase; SCA26

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.