Rabbit anti-EFTUD1 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
EFTUD1 is involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SBDS, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Has low intrinsic GTPase activity. GTPase activity is increased by contact with 60S ribosome subunits. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q7Z2Z2].
Alternate Names
EFL1; EFTUD1; elongation factor Tu GTP binding domain containing 1; elongation factor Tu GTP-binding domain-containing protein 1; elongation factor-like 1; elongation factor-like GTPase 1; FAM42A; HsT19294; RIA1; ribosome assembly 1 homolog; SDS2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.