Rabbit anti-EFTUD2/SNRP116 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-957A maps to a region between residue 1 and 50 of human Elongation Factor Tu GTP Binding Domain Containing 2 (U5 snRNP-Specific Protein, 116 kD ) using the numbering given in entry NP_004238.2 (GeneID 9343).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to EFTUD2/SNRP116 immobilized on solid support.
The epitope recognized by A300-957A-T maps to a region between residue 1 and 50 of human Elongation Factor Tu GTP Binding Domain Containing 2 (U5 snRNP-Specific Protein, 116 kD ) using the numbering given in entry NP_004238.2 (GeneID 9343).
Additional Product Information
The splicesome is the molecular machine responsible for catalyzing intron removal from pre-mRNA transcripts. The splicesome is composed of several small nuclear RNAs (snRNAs) complexed with their associated proteins [together termed snRNPs (small nuclear ribonucleoprotein)]. One of the major classes of snRNPs includes the U1, U2, U4, U5 and U6 snRNP. EFTUD2/SNRP116 is a component of the U5 snRNP. EFTUD2/SNRP116 is a GTPase that may play a role in disrupting U4/U6 helices prior to splicesome activation.
Alternate Names
116 kDa U5 small nuclear ribonucleoprotein component; elongation factor Tu GTP-binding domain-containing protein 2; hSNU114; MFDGA; MFDM; SNRNP116; Snrp116; Snu114; SNU114 homolog; U5 snRNP-specific protein, 116 kDa; U5-116 kDa; U5-116KD
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.