Rabbit anti-EGR1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-390A maps to a region between residue 1 and 50 of mouse Early Growth Response Protein 1 using the numbering given in entry NP_031939.1 (GeneID 13653).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to EGR1 immobilized on solid support.
The epitope recognized by A303-390A-T maps to a region between residue 1 and 50 of mouse Early Growth Response Protein 1 using the numbering given in entry NP_031939.1 (GeneID 13653).
Additional Product Information
Early growth response protein 1 (EGR1) is a transcriptional regulator that recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). EGR1 activates the transcription of target genes whose products are required for mitogenesis and differentiation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P08046].
Alternate Names
A530045N19Rik; early growth response protein 1; egr; Egr-1; ETR103; Krox-1; Krox24; Krox-24; Krox-24 nuclear protein; nerve growth factor-induced protein A; NGF1-A; NGFIA; NGFI-A; TIS8; transcription factor Zif268; Zenk; Zfp-6; Zif268; zinc finger protein Krox-24
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.